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Image Search Results
Journal: Journal of immunology (Baltimore, Md. : 1950)
Article Title: The regulation of inherently autoreactive VH4-34-expressing B cells in individuals living in a malaria endemic area of West Africa
doi: 10.4049/jimmunol.1600491
Figure Lengend Snippet: Characterization of 9G4+ B cells in the peripheral blood of US adults and Malian adults and children. (A) Percent of CD19+ B cells that are 9G4+ in the PBMCs of U.S. adults and Malian adults and six year old children. Mean and standard deviation (SD) are shown. (B) Percent of all CD19+ B cells that are naïve (IgD+, CD27−), unswitched CD27+ (IgD+, CD27+), classical switched MBCs (IgD−, CD27+), or switched, CD27− B cells (IgD−, CD27−). Mean and standard error of the mean (SEM) are shown. (C) Percent of B cells in each subset shown in B that are9G4+. Mean and SEM are shown. (D) Percent of 9G4+ B cells that stained using anti-IgM, anti-IgD, anti-IgG or anti-IgA or were not stained by these reagents (Other). Mean and SEM are shown. (E–F) Percent of all CD19+ B cell in each subset (All) or percent of 9G4+ B cell in each subset (9G4+) that were CD21− for U.S. adults (E) and Malian adults (F). Mean and SD are shown. Statistical comparisons were by Student’s T-test (* = p-value < 0.05; ** = p-value < 0.005; *** = p-value < 0.0005).
Article Snippet: Eleven serial 2-fold dilutions of either
Techniques: Standard Deviation, Staining
Journal: Journal of immunology (Baltimore, Md. : 1950)
Article Title: The regulation of inherently autoreactive VH4-34-expressing B cells in individuals living in a malaria endemic area of West Africa
doi: 10.4049/jimmunol.1600491
Figure Lengend Snippet: Somatic hypermutation analysis of Malian young children and adult VH. (A) IgH sequence analysis for PBMCs acquired from 6 infants (3–11 months) and 9 toddlers (13–42 months) prior to the malaria transmission season (Pre) and 17 days following acute febrile malaria (Acute). Top panel. Average number of mutations in IgM-, IgG- and IgA-VH4-34. Bottom panel. Average number of mutations in IgM, IgG and IgA for all other VH genes. Statistical comparisons were by Mann Whitney U Test (ns = p value > 0.05; * = p value ≤ 0.05; ** = p value ≤ 0.01; *** = p value ≤ 0.001; **** = p value ≤ 0.0001). (B) Number of non-gap VH mutations for IgM and both IgG and IgA for naïve B cells, classical MBCs and atypical MBCs purified from the PBMCs acquired from four Malian adults for VH4-34 and all other VH.
Article Snippet: Eleven serial 2-fold dilutions of either
Techniques: Sequencing, Transmission Assay, MANN-WHITNEY, Purification
Journal: Journal of immunology (Baltimore, Md. : 1950)
Article Title: The regulation of inherently autoreactive VH4-34-expressing B cells in individuals living in a malaria endemic area of West Africa
doi: 10.4049/jimmunol.1600491
Figure Lengend Snippet: Correlation of 9G4+ IgG levels and protection from acute febrile malaria. Seventy-four seven to nine year old children (nine seven year olds; 51 eight year olds; 14 nine year olds) were grouped as protected or not from acute malaria and either PCR+ for malaria parasites or not before the malaria season. (A). 9G4+-IgG levels in May at the end of the dry season prior to the malaria transmission season. Medians are shown. Statistical comparisons were by Mann-Whitney test. (* = p < 0.05) (B) Total IgG levels in May at the end of the dry season prior to the malaria transmission season. Medians are shown. Statistical comparisons were by Mann-Whitney test. (* = p < 0.05) (C) Multiple logistic regression testing whether 9G4+ IgG or 9G4+ IgM levels at the end of the dry season predicted resistance to acute febrile malaria during the ensuing malaria transmission season. Logistic regression was fit using JMP11.
Article Snippet: Eleven serial 2-fold dilutions of either
Techniques: Transmission Assay, MANN-WHITNEY